A method of reducing the dose during the radiation sterilization process of bone grafts

Among all the technologies of bone implant sterilization the most widely used is the radiation sterilization method with gamma rays or electrons. The determining parameter is the value of the absorbed dose. Dose established by the IAEA for sterilization of bone grafts is 25 kGy. One of the largest problems is the dose reduction while preservation of high sterility of the implants. In order to overcome the above drawbacks, an additional sterilizing factor was proposed to use as a sterilization method comprising treating the implant in two steps with an ozone-air mixture, which reduces radioresistance and reduces the population of microorganisms before subsequent radiation processing. Microbiological studies have confirmed the effectiveness of the developed method.